OcellO uses co-cultures of HLA-matched tumour cells and immune cells to test the effects of compounds on the cytotoxic activity of T cells. Image-based measurement of selected features (eg. total tumor volume; number, shape and size of tumor cells; number, shape and size of tumor cell nuclei) yields robust, reproducible and high-throughput (384 well) results.

 

Killing protocol

We offer:
infiltrationkilling

Advantages:

•       Physiologically relevant environment

•       Visualization of T cell-cancer cell interactions

•       Automated analysis and solid quantification of killing capacity

•       HLA-matched cellular players

•       Can be combined with infiltration assay

 

Determination of cytotoxic capacity

killing graphsPBMCs were co-cultured with breast cancer tumoroids in the presence or absence of T cell activators. Tumoroid volumes were measured.

For more information contact us at info@ocello.nl.