OcellO specialises in generating models that closely recapitulate both healthy and disease phenotypes. Healthy tissues show normal functions, such as salt and water transport in kidney epithelial tubules or production of milk proteins by breast epithelial cells – functions that are lost in 2D cultures.  In our disease models, tissues also show the expected disruption of phenotype and function that is associated with patient disease; cancer cells demonstrate dysplasia and become invasive, whereas epithelial cells from patients with cystic diseases show abnormal cyst formation. A more accurate simulation of the in vivo disease phenotype allows you to select more better quality molecules to progress through your discovery pipeline.

 

Using our disease models we can obtain various functional and clinical relevant readouts

  • Proliferation, Migration and invasion
  • apico-basal polarity, dysplasia, lumen formation
  • Cyst swelling and transporter function

Over 150 models are available for screening of your compounds:
Overview types of assays large

  • It is also possible to screen your compounds in panels of assays:
    Panel of 30 patient-derived colorectal cancer and wt-organoids
  • Panel of 50 breast cancer cell lines
  • Lung, prostate and kidney carcinomas, osteosarcoma

In these models we can show whether the candidate compounds:

  • Restore the disease phenotype to a healthy state
  • Reduce the malignancy of cancer cells (inhibit invasion, inhibt growth or kill cells)
  • Increase sensitivity to existing anti-cancer therapies (drug combinations)

Advantage of testing compounds in a disease model:

  • Unbiased testing
  • Possibilities to identify new classes of compounds
  • Possibilities to identify new targets and pathways